| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Roy, Sylvia M (12)
|
AMMLAC |
1:01.65 |
1:17.32 |
15.67 |
| 2 |
Ricciuti, Giuliana L (12)
|
AMSPY |
1:25.37 |
1:21.43 |
-3.94 |
| 3 |
Shipe, Dylayn (11)
|
AMUN-R |
1:26.55 |
1:27.86 |
1.31 |
| 4 |
Spiess, Emma E (12)
|
AMHD |
1:26.13 |
1:28.68 |
2.55 |
| 5 |
Fraundorfer, Alexis R (12)
|
AMBRY |
1:12.83 |
1:29.29 |
16.46 |
| 6 |
Shipe, Bryony (11)
|
AMUN-R |
1:27.92 |
1:29.96 |
2.04 |
| 7 |
Edner, Callahan E (12)
|
AMBPR |
1:18.28 |
1:31.53 |
13.25 |
| 8 |
Morian, Megan E (12)
|
AMBPR |
1:30.84 |
1:32.18 |
1.34 |
| 9 |
Pazuchanics, Kathryn E (12)
|
AMPTSC |
1:15.60 |
1:33.32 |
17.72 |
|
Berg, Madeline M (12)
|
AMSFAC |
1:23.96 |
DQ |
- |