| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Myers, Grace E (16)
|
AMBPR |
2:33.71 |
2:31.62 |
-2.09 |
| 2 |
Baiano, Gabriella L (15)
|
AMSFAC |
2:39.67 |
2:31.77 |
-7.90 |
| 3 |
Ransom, Janiya A (17)
|
AMWAAC |
2:32.51 |
2:33.68 |
1.17 |
| 4 |
Jackovic, Emily A (16)
|
AMSPAC |
2:44.57 |
2:39.51 |
-5.06 |
| 5 |
Hayson, Jenna N (15)
|
AMRACE |
2:41.31 |
2:40.04 |
-1.27 |
| 6 |
Zukina, Bernadette L (15)
|
AMNAC |
2:36.35 |
2:40.81 |
4.46 |
| 7 |
Jozwiak, Rebecca J (15)
|
AMMLAC |
|
2:42.25 |
- |
| 8 |
Webster, Olivia L (18)
|
AMWAAC |
2:46.39 |
2:46.70 |
0.31 |
| 9 |
Augustynowicz, Mara K (15)
|
AMPTSC |
3:16.89 |
2:51.53 |
-25.36 |
| 10 |
Schwertfuehrer, Molly K (15)
|
AMMLAC |
3:45.99 |
2:54.00 |
-51.99 |