| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Webster, Gabrielle L (15)
|
AMWAAC |
29.20 |
29.47 |
0.27 |
| 2 |
Ransom, Janiya A (17)
|
AMWAAC |
30.47 |
30.63 |
0.16 |
| 3 |
Zukina, Bernadette L (15)
|
AMNAC |
32.22 |
31.25 |
-0.97 |
| 4 |
Myers, Grace E (16)
|
AMBPR |
31.47 |
31.68 |
0.21 |
| 5 |
Baiano, Gabriella L (15)
|
AMSFAC |
31.28 |
32.01 |
0.73 |
| 6 |
Gitelman, Rachel T (16)
|
AMPEAQ |
30.19 |
32.07 |
1.88 |
| 7 |
Valli, Vanesa V (15)
|
AMRACE |
32.60 |
32.36 |
-0.24 |
| 8 |
Jackovic, Emily A (16)
|
AMSPAC |
35.05 |
33.11 |
-1.94 |
| 9 |
Smith, Emma C (15)
|
AMMOON |
31.77 |
33.46 |
1.69 |
| 10 |
Evans, Danika A (17)
|
AMSPAC |
34.76 |
34.00 |
-0.76 |
| 11 |
Webster, Olivia L (18)
|
AMWAAC |
34.88 |
35.29 |
0.41 |
| 12 |
Augustynowicz, Mara K (15)
|
AMPTSC |
35.46 |
35.42 |
-0.04 |
| 13 |
Jozwiak, Rebecca J (15)
|
AMMLAC |
34.49 |
35.93 |
1.44 |
| 14 |
Schwertfuehrer, Molly K (15)
|
AMMLAC |
36.99 |
37.48 |
0.49 |
| 15 |
Shriver, Suaysawan (17)
|
AMMLAC |
45.99 |
40.80 |
-5.19 |