| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Houser, McKenna L (13)
|
AMMOON |
3:15.48 |
3:16.31 |
0.83 |
| 2 |
Munoz, Kirsten D (13)
|
AMSFAC |
3:17.55 |
3:20.10 |
2.55 |
| 3 |
Brugger, Ellie A (13)
|
AMPWRS |
3:23.26 |
3:20.44 |
-2.82 |
| 4 |
Walko, Julia F (13)
|
AMNAC |
3:22.65 |
3:23.08 |
0.43 |
| 5 |
Brown, Angelina R (14)
|
LELESD |
3:27.11 |
3:26.88 |
-0.23 |
| 6 |
Newton, Madisen G (13)
|
AMSVSC |
3:36.27 |
3:28.12 |
-8.15 |
| 7 |
Arendas, Emily T (13)
|
AMNAC |
3:19.54 |
3:32.01 |
12.47 |
| 8 |
Reinhart, Mary Elson (Ellie) (13)
|
AMMLAC |
3:41.27 |
3:34.30 |
-6.97 |
| 9 |
Tan, Amy Y (14)
|
AMMLAC |
3:22.99 |
3:40.03 |
17.04 |
| 10 |
Kile, Fernanda F (13)
|
AMPEAQ |
3:25.39 |
3:41.27 |
15.88 |
| 11 |
Dunn, Riley N (14)
|
AMTRIN |
3:40.53 |
3:41.79 |
1.26 |
| 12 |
Osullivan, Avery D (13)
|
AMTRIN |
3:57.94 |
4:02.17 |
4.23 |
| 13 |
Abraham, Elizabeth S (13)
|
AMWAAC |
3:36.88 |
4:04.97 |
28.09 |
| 14 |
Bixler, Willow T (14)
|
AMFAST |
3:53.79 |
4:06.43 |
12.64 |
|
MacAdams, Annabelle M (13)
|
AMPEAQ |
3:03.46 |
NS |
- |