| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Gotaskie, Mary C (12)
|
AMJCCS |
2:25.87 |
2:21.76 |
-4.11 |
| 2 |
Ricciuti, Giuliana L (12)
|
AMSPY |
2:34.25 |
2:31.42 |
-2.83 |
| 3 |
Huang, Rayna L (12)
|
AMKW |
2:35.72 |
2:31.87 |
-3.85 |
| 4 |
Hull, Magdalena J (11)
|
AMFAST |
2:36.04 |
2:34.55 |
-1.49 |
| 5 |
Oates, Amelia M (12)
|
AMFAST |
2:49.14 |
2:38.42 |
-10.72 |
| 6 |
Haas, Abigail D (11)
|
AMKW |
2:43.99 |
2:39.37 |
-4.62 |
| 7 |
Price, Brooke A (11)
|
AMSPY |
2:46.46 |
2:45.76 |
-0.70 |
| 8 |
Kovach, Grace E (12)
|
AMKW |
2:53.13 |
2:47.43 |
-5.70 |
| 9 |
Klingensmith, Hanna (12)
|
AMNAC |
2:44.54 |
2:49.07 |
4.53 |
| 10 |
Piraino, Maria A (12)
|
AMFAST |
3:05.04 |
2:50.42 |
-14.62 |
| 11 |
Hwang, Moonyoung (12)
|
AMKW |
3:45.02 |
2:59.53 |
-45.49 |
| 12 |
Marculescu, Cristina (12)
|
AMKW |
3:14.53 |
3:00.34 |
-14.19 |
| 13 |
Sullivan, Riley A (12)
|
AMNAC |
3:05.84 |
3:01.84 |
-4.00 |
| 14 |
Barle, Josephine V (12)
|
AMNAC |
3:03.68 |
3:02.46 |
-1.22 |
| 15 |
Resek, Madison E (12)
|
AMKW |
3:01.75 |
3:05.78 |
4.03 |
| 16 |
Laurenti, Gianna M (11)
|
AMMMSA |
3:20.99 |
3:08.24 |
-12.75 |
| 17 |
Byars, Adeline F (11)
|
AMNAC |
3:36.54 |
3:20.83 |
-15.71 |
|
Stanczak, Josephine E (11)
|
AMKW |
3:19.85 |
DQ |
- |
|
Kunkle, Paige K (12)
|
AMFAST |
2:34.65 |
NS |
- |
|
Hilty, Savanna J (11)
|
AMFAST |
1:42.99 |
NS |
- |