| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Brook, Channing C (14)
|
AMKW |
2:40.45 |
2:46.44 |
5.99 |
| 2 |
Wen, Gloria Y (13)
|
AMKW |
2:51.39 |
2:48.58 |
-2.81 |
| 3 |
Lowe, Kate E (13)
|
AMFAST |
2:46.39 |
2:50.30 |
3.91 |
| 4 |
Gaydos, Rosemary E (13)
|
AMNAC |
2:54.72 |
2:52.04 |
-2.68 |
| 5 |
Schollaert, Therese A (13)
|
AMSPY |
3:03.48 |
2:52.17 |
-11.31 |
| 6 |
McFaden, Amanda J (14)
|
AMJCCS |
2:36.41 |
2:52.66 |
16.25 |
| 7 |
LaRoche, Francesca L (14)
|
AMWHAT |
2:58.95 |
2:54.41 |
-4.54 |
| 8 |
Walko, Julia F (14)
|
AMNAC |
3:00.35 |
2:55.77 |
-4.58 |
| 9 |
Rusche, Margaret M (13)
|
AMKW |
3:20.02 |
2:56.77 |
-23.25 |
| 10 |
Ricciuti, Nina M (14)
|
AMSPY |
2:59.83 |
2:57.29 |
-2.54 |
| 11 |
Harris, Emma R (13)
|
AMPTAC |
2:57.14 |
2:57.52 |
0.38 |
| 12 |
Fraundorfer, Alexis R (13)
|
AMBRY |
2:59.41 |
2:58.28 |
-1.13 |
| 13 |
Black, Chloe R (13)
|
AMFAST |
2:52.06 |
2:59.01 |
6.95 |
| 14 |
Arendas, Emily T (13)
|
AMNAC |
2:57.54 |
3:01.71 |
4.17 |
| 15 |
Innis, Sasha E (13)
|
AMGJY |
3:05.10 |
3:01.83 |
-3.27 |
| 16 |
Haas, Katherine R (13)
|
AMKW |
2:59.86 |
3:02.00 |
2.14 |
| 17 |
Holmes, Morgan S (14)
|
AMMMSA |
2:50.12 |
3:02.47 |
12.35 |
| 18 |
McCandless, Sarah N (14)
|
AMSRST |
3:14.87 |
3:07.48 |
-7.39 |
| 19 |
Arendas, Allison N (13)
|
AMNAC |
3:16.54 |
3:07.50 |
-9.04 |
| 20 |
Tucker, Angela M (14)
|
AMCSC |
3:17.84 |
3:13.19 |
-4.65 |
| 21 |
Plank, Anna R (14)
|
AMSRST |
3:25.23 |
3:13.32 |
-11.91 |
| 22 |
Hunter, Lillian (14)
|
AMMPAC |
2:58.79 |
3:16.27 |
17.48 |
| 23 |
Bixler, Willow T (14)
|
AMFAST |
3:22.44 |
3:21.03 |
-1.41 |
| 24 |
Huang, Grace S (13)
|
AMFAST |
3:24.99 |
3:21.47 |
-3.52 |
| 25 |
Tedesco, Emma J (13)
|
AMJCCS |
3:35.14 |
3:21.92 |
-13.22 |
| 26 |
Fillar, Kayla R (13)
|
AMNAC |
3:40.43 |
3:24.87 |
-15.56 |
| 27 |
Matrisch, Saige M (13)
|
AMPTAC |
3:31.56 |
3:28.71 |
-2.85 |
|
Pratt, Riley M (14)
|
AMKW |
2:45.78 |
NS |
- |
|
O'Toole, Payton E (14)
|
AMKW |
2:46.23 |
NS |
- |