| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Kelley, Maeve E (14)
|
AMKW |
2:15.94 |
2:21.01 |
5.07 |
| 2 |
Brook, Channing C (14)
|
AMKW |
2:16.29 |
2:21.07 |
4.78 |
| 3 |
Eiben, Natalie J (14)
|
AMJCCS |
2:18.06 |
2:23.16 |
5.10 |
| 4 |
McFaden, Amanda J (14)
|
AMJCCS |
2:18.64 |
2:25.66 |
7.02 |
| 5 |
Schollaert, Therese A (13)
|
AMSPY |
2:23.30 |
2:26.94 |
3.64 |
| 6 |
O'Toole, Payton E (14)
|
AMKW |
2:23.17 |
2:27.50 |
4.33 |
| 7 |
Hilty, Allyson N (14)
|
AMFAST |
2:21.50 |
2:27.93 |
6.43 |
| 8 |
Holmes, Morgan S (14)
|
AMMMSA |
2:20.53 |
2:28.57 |
8.04 |
| 9 |
Wen, Gloria Y (13)
|
AMKW |
2:34.12 |
2:30.29 |
-3.83 |
| 10 |
Rusche, Margaret M (13)
|
AMKW |
2:29.33 |
2:30.71 |
1.38 |
| 11 |
King, Abigail (13)
|
AMCSC |
2:31.09 |
2:31.18 |
0.09 |
| 12 |
LaRoche, Francesca L (14)
|
AMWHAT |
2:32.91 |
2:33.73 |
0.82 |
| 14 |
Black, Chloe R (13)
|
AMFAST |
2:28.57 |
2:34.78 |
6.21 |
| 15 |
Vogelsang, Bryn A (14)
|
AMGLAC |
2:34.66 |
2:37.79 |
3.13 |
| 16 |
Walko, Julia F (14)
|
AMNAC |
2:40.26 |
2:39.43 |
-0.83 |
| 17 |
Cagle, Elise G (13)
|
AMSRST |
2:41.31 |
2:39.85 |
-1.46 |
| 18 |
Murphy, Sierra E (14)
|
AMMMSA |
2:42.12 |
2:40.82 |
-1.30 |
| 19 |
Estremera, Michelina S (13)
|
AMMMSA |
2:44.63 |
2:42.28 |
-2.35 |
| 20 |
Van Deusen, Gretchen P (13)
|
AMGPAC |
2:45.91 |
2:43.18 |
-2.73 |
| 21 |
Bonavita, Emily (13)
|
AMJCCS |
2:48.97 |
2:43.54 |
-5.43 |
| 22 |
Haas, Katherine R (13)
|
AMKW |
2:42.11 |
2:44.69 |
2.58 |
| 23 |
Harris, Emma R (13)
|
AMPTAC |
3:04.70 |
2:45.44 |
-19.26 |
| 24 |
Bixler, Willow T (14)
|
AMFAST |
2:51.37 |
2:45.54 |
-5.83 |
| 25 |
Johnson, Olivia J (14)
|
AMPTAC |
2:46.79 |
2:48.19 |
1.40 |
| 26 |
Kohan, Karlee E (13)
|
AMGJY |
2:38.92 |
2:50.36 |
11.44 |
| 27 |
Wienand, Paige L (13)
|
AMMMSA |
3:00.46 |
2:51.64 |
-8.82 |
| 28 |
Bishop, Emma G (13)
|
AMPTAC |
2:48.29 |
2:51.72 |
3.43 |
| 29 |
DeVito, Mia (13)
|
AMCSC |
2:43.45 |
2:51.84 |
8.39 |
| 30 |
Arendas, Emily T (13)
|
AMNAC |
2:58.67 |
2:53.47 |
-5.20 |
| 31 |
Siri, Nora (13)
|
AMKW |
3:30.02 |
2:54.75 |
-35.27 |
| 32 |
Tucker, Angela M (14)
|
AMCSC |
3:02.97 |
2:56.90 |
-6.07 |
| 33 |
Reed, Ella A (13)
|
AMJCCS |
3:09.48 |
2:59.73 |
-9.75 |
| 34 |
Fillar, Kayla R (13)
|
AMNAC |
3:04.16 |
3:02.14 |
-2.02 |
| 35 |
Huang, Grace S (13)
|
AMFAST |
3:16.75 |
3:10.56 |
-6.19 |
|
Wang, Grace L (14)
|
AMFAST |
2:28.50 |
DQ |
- |
|
Marcos, Isabella S (13)
|
AMKW |
2:23.89 |
NS |
- |
|
Pratt, Riley M (14)
|
AMKW |
2:33.36 |
NS |
- |