| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Hunt, Santina A (9)
|
SRBSC |
46.21 |
42.38 |
-3.83 |
| 2 |
Paucar, Bellazlin H (10)
|
SRSV5B |
51.95 |
54.69 |
2.74 |
| 3 |
Nickel, Brooklynn A (9)
|
SRBSC |
57.25 |
54.87 |
-2.38 |
| 4 |
Stoddard, Penny L (9)
|
SRBSC |
|
55.75 |
- |
| 5 |
Meckelson, Kayla M (9)
|
SRNST |
|
1:04.17 |
- |
|
Brady, Lillian M (10)
|
SRBY |
|
DQ |
- |
|
Edmonds, Lily E (10)
|
SRBY |
|
DQ |
- |
|
Leslie, Campbell J (9)
|
SRBY |
|
DQ |
- |
|
Pittard, Eliana C (9)
|
SRBY |
1:07.28 |
DQ |
- |
|
Sherburne, Juniper R (9)
|
SRBY |
|
DQ |
- |
|
Swift, Teaella G (10)
|
SRBY |
|
DQ |
- |
|
Larson, Savannah R (10)
|
SREST |
1:21.58 |
DQ |
- |
|
Bell, Katie J (10)
|
SRNST |
|
DQ |
- |
|
Buchinskiy, Elizabeth (9)
|
SRNST |
|
DQ |
- |