| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Fenn, Lauren E (14)
|
MAPFY |
26.21 |
27.07 |
0.86 |
| 1 |
Delaney, Julia J (13)
|
MAPFY |
29.38 |
28.49 |
-0.89 |
| 1 |
Kleber, Lydia C (13)
|
NJUN-4 |
|
28.56 |
- |
| 2 |
Moskowitz, Jennifer E (14)
|
NJUN-2 |
27.06 |
27.21 |
0.15 |
| 2 |
Callahan, Chloe K (13)
|
MAUN-W |
|
29.43 |
- |
| 2 |
McCall, Corinne C (13)
|
MAPFY |
30.37 |
29.85 |
-0.52 |
| 3 |
Roesch, Raquelle S (14)
|
MABLUE |
25.49 |
27.54 |
2.05 |
| 3 |
Heard, Adeline M (13)
|
MABLUE |
|
29.57 |
- |
| 3 |
Pedraza, Isabella (14)
|
MAPJA |
30.24 |
29.88 |
-0.36 |
| 4 |
Blazure, Mckayla (13)
|
MAPJA |
30.89 |
30.03 |
-0.86 |
| 4 |
Tegler, Brittney A (14)
|
MAWAP |
30.38 |
30.54 |
0.16 |
| 5 |
Theisen, Olivia V (13)
|
MAUN-P |
|
31.03 |
- |
| 6 |
Allena, Isabella R (14)
|
MABLUE |
45.55 |
31.18 |
-14.37 |
| 7 |
McCloskey, Madison R (13)
|
MAUN-B |
33.20 |
32.70 |
-0.50 |
| 8 |
Palmer, Arden L (13)
|
MAEAST |
32.75 |
32.80 |
0.05 |
| 9 |
Tegler, Lindsey C (13)
|
MAWAP |
34.19 |
34.40 |
0.21 |