| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Xia, Elizabeth (16)
|
CTNCY |
25.33 |
27.43 |
2.10 |
| 2 |
Hendricks, Carolyn R (17)
|
CTNCY |
25.75 |
27.51 |
1.76 |
| 3 |
Peloso, Emma L (14)
|
CTNCY |
25.98 |
27.61 |
1.63 |
| 4 |
Dunn, Lily M (14)
|
CTNCY |
26.45 |
28.03 |
1.58 |
| 5 |
Jisl, Katarina P (16)
|
CTNCY |
27.33 |
28.15 |
0.82 |
| 6 |
Williams, Emma A (14)
|
CTNCY |
28.47 |
28.57 |
0.10 |
| 7 |
Carrillo, Alexa A (17)
|
CTNCY |
28.16 |
29.41 |
1.25 |
| 8 |
Johansson, Eve P (16)
|
CTNCY |
29.38 |
29.65 |
0.27 |
| 9 |
Lee, Skylar A (15)
|
CTNCY |
27.74 |
29.84 |
2.10 |
|
Laurence, Charlotte L (14)
|
CTNCY |
27.86 |
NS |
- |
|
Trygg, Estella E (16)
|
CTNCY |
28.46 |
NS |
- |