| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Wilson, Edmund (16)
|
MRRFAC |
23.61 |
23.49 |
-0.12 |
| 2 |
Landgraf, Tim A (17)
|
MRRFAC |
23.41 |
23.51 |
0.10 |
| 3 |
Gerbes, Jackson E (17)
|
MRNBS |
24.25 |
24.17 |
-0.08 |
| 4 |
Mummery, John F (15)
|
MRNBS |
23.99 |
24.30 |
0.31 |
| 5 |
Tirch, Patrick M (17)
|
MRHVD |
23.88 |
24.32 |
0.44 |
| 6 |
Velasquez, Vicente O (16)
|
MRNBS |
23.47 |
24.55 |
1.08 |
| 7 |
Reade, Angelo R (16)
|
MRNBS |
23.32 |
24.80 |
1.48 |
| 8 |
Karadeniz, Derin M (15)
|
MRHVD |
25.14 |
25.15 |
0.01 |
| 9 |
Tuttle, Peyton J (15)
|
MRNBS |
25.21 |
25.23 |
0.02 |
| 10 |
Del Rosario, Edmund Jacob C (15)
|
MRPATS |
25.16 |
25.24 |
0.08 |
| 11 |
Cooper, Jackson C (15)
|
MRNBS |
24.76 |
25.27 |
0.51 |
| 12 |
Kyaw, Thit-sar (15)
|
MRHVD |
25.87 |
25.34 |
-0.53 |
| 13 |
Burgarelli, Sebastian (15)
|
MRNBS |
26.58 |
26.34 |
-0.24 |
| 14 |
Davis, Miles K (15)
|
MRNBS |
26.44 |
26.65 |
0.21 |
| 15 |
Juarez Espinoza, Marvin (17)
|
MRMHHW |
28.07 |
27.67 |
-0.40 |
| 16 |
Constancio, Markus A (15)
|
MRNBS |
29.75 |
30.81 |
1.06 |