| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Williams, Grace K (12)
|
MRNBS |
42.12 |
40.20 |
-1.92 |
| 2 |
MacHugh, Grace A (12)
|
MRNBS |
45.10 |
41.63 |
-3.47 |
| 3 |
Dineen, Molly (11)
|
MRMWSC |
42.83 |
43.64 |
0.81 |
| 4 |
Mayerhauser, Lea E (11)
|
MRDA |
46.99 |
45.92 |
-1.07 |
| 5 |
Bunch, Jenna G (11)
|
MRNBS |
46.77 |
46.70 |
-0.07 |
| 6 |
Kaw, Rhea (12)
|
MRMWSC |
|
46.71 |
- |
| 7 |
Kaw, Sheena (12)
|
MRMWSC |
|
48.02 |
- |
| 8 |
Regenauer, Katarina T (11)
|
MRNBS |
49.42 |
48.56 |
-0.86 |
| 9 |
Donnellan, Molly E (12)
|
MRMWSC |
47.09 |
49.08 |
1.99 |
| 10 |
Sheerin, Megan N (11)
|
MRMWSC |
49.38 |
50.52 |
1.14 |
| 11 |
Kohlbecker, Sophia D (12)
|
MRMWSC |
52.47 |
51.50 |
-0.97 |
| 12 |
Moffit, Maya B (11)
|
MRDA |
55.87 |
54.42 |
-1.45 |
| 13 |
Weeks, Kelly P (11)
|
MRUNNB |
|
55.40 |
- |
|
Bilenky, Adriana (11)
|
MRNBS |
57.67 |
DQ |
- |
|
Mendoza, Emily (12)
|
MRNBS |
|
DQ |
- |