| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Fenska, Allison P (16)
|
MAJW |
23.39 |
24.47 |
1.08 |
| 2 |
Feuzou Mekoue, Kelly (15)
|
MASSC |
23.99 |
24.56 |
0.57 |
| 3 |
Paglia, Sydney J (17)
|
MASSC |
23.74 |
24.69 |
0.95 |
| 4 |
Famous, Caroline A (18)
|
MASSC |
22.97 |
24.70 |
1.73 |
| 5 |
Behm, Ann E (16)
|
MAJW |
24.57 |
25.26 |
0.69 |
| 6 |
Liu, Chelsey (17)
|
MASSC |
24.03 |
25.28 |
1.25 |
| 7 |
Kaczorowski, Gabriella A (15)
|
MAJW |
25.32 |
25.88 |
0.56 |
| 8 |
McGovern, Emma L (16)
|
MASSC |
26.10 |
25.90 |
-0.20 |
| 9 |
Barber, Avery S (16)
|
MASSC |
25.23 |
26.02 |
0.79 |
| 10 |
Mazon, Madison H (17)
|
MASSC |
25.08 |
26.14 |
1.06 |
| 11 |
Pujadas, Riley E (17)
|
MASSC |
25.19 |
26.32 |
1.13 |
| 12 |
Shuff, Darrah L (14)
|
MAJW |
25.04 |
26.50 |
1.46 |
| 13 |
Jensen, Erin E (17)
|
MAJW |
25.77 |
26.79 |
1.02 |
| 14 |
Jones, Laura G (17)
|
MASSC |
25.43 |
26.87 |
1.44 |
| 15 |
Ott, Madeleine F (16)
|
MASSC |
26.01 |
27.04 |
1.03 |
| 16 |
Jones, Alexandra S (17)
|
MASSC |
27.32 |
27.25 |
-0.07 |
| 17 |
Stevens, Molly N (16)
|
MASSC |
25.77 |
27.28 |
1.51 |
|
Hendrickson, Ava E (15)
|
MASSC |
27.75 |
NS |
- |