| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Bloom, Taylor L (11)
|
NCUN-T |
2:35.96 |
2:28.00 |
-7.96 |
| 2 |
Frost, Hope C (12)
|
VADIG |
2:31.41 |
2:35.47 |
4.06 |
| 3 |
Alinsonorin, Bianca C (12)
|
VATAC |
2:49.60 |
2:37.02 |
-12.58 |
| 4 |
Barnes, Payton E (11)
|
NCTAC |
2:45.49 |
2:37.72 |
-7.77 |
| 5 |
Starks, Brynn M (12)
|
VADIG |
2:32.66 |
2:38.62 |
5.96 |
| 6 |
Tjandra, Samantha E (11)
|
NCTAC |
2:45.87 |
2:38.70 |
-7.17 |
| 7 |
Allred, Samantha R (12)
|
NCTAC |
2:49.26 |
2:39.27 |
-9.99 |
| 8 |
Curran, Molly D (11)
|
NCTAC |
2:45.58 |
2:41.44 |
-4.14 |
| 9 |
Perkins, Hazel G (11)
|
NCTAC |
3:08.84 |
2:41.64 |
-27.20 |
| 10 |
Ashby, Leah P (12)
|
VADIG |
3:02.95 |
2:44.18 |
-18.77 |
| 11 |
Nance, Lauren M (11)
|
NCTAC |
2:55.75 |
2:46.46 |
-9.29 |
| 12 |
Valent, Cameron C (12)
|
NCTAC |
3:02.84 |
2:47.10 |
-15.74 |
| 13 |
Baker, Skye A (12)
|
VATAC |
3:04.69 |
2:49.00 |
-15.69 |
| 14 |
Boughton, Grace M (11)
|
NCTAC |
3:04.06 |
2:51.64 |
-12.42 |
| 15 |
Mitchell, Aliya R (12)
|
NCTAC |
3:00.72 |
2:54.45 |
-6.27 |
| 16 |
Besachio, Alessandra L (12)
|
VATAC |
|
2:57.41 |
- |
|
Britt, Emily M (11)
|
VATAC |
3:27.70 |
DQ |
- |
|
Becker, Kailyn R (11)
|
NCUN-T |
3:16.89 |
DQ |
- |
|
Benkusky, Gwyneth A (12)
|
VAOBX |
|
DQ |
- |
|
Newcome, Ava L (12)
|
NCTAC |
2:51.16 |
NS |
- |