| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Broedell, Tessa M (10)
|
FGJDST |
30.93 |
31.18 |
0.25 |
| 2 |
Smithwick, Myriam L (10)
|
FGJDST |
31.43 |
32.06 |
0.63 |
| 3 |
Polston, Olivia E (10)
|
FGWOW |
35.25 |
34.04 |
-1.21 |
| 4 |
Morgan, Melea R (9)
|
FGJDST |
34.22 |
35.07 |
0.85 |
| 5 |
Tiscione, Eden G (9)
|
FGWOW |
39.70 |
37.72 |
-1.98 |
| 6 |
Tribou, Miriam E (10)
|
FGJDST |
42.03 |
38.96 |
-3.07 |
| 7 |
Rusczyk, Caroline E (10)
|
FGJDST |
41.37 |
42.22 |
0.85 |
| 8 |
Cihowiak, Ava E (9)
|
FGJDST |
44.07 |
42.37 |
-1.70 |
| 9 |
Roman, Madalyn R (9)
|
FGLLL |
39.27 |
44.86 |
5.59 |
| 10 |
Phan, Emma U (9)
|
FGJDST |
51.24 |
47.28 |
-3.96 |
| 11 |
Branstetter, Madyson A (10)
|
FGLLL |
57.24 |
49.60 |
-7.64 |
| 12 |
Brown, Daniela Z (9)
|
FGLLL |
46.57 |
49.79 |
3.22 |
| 13 |
Velez, Camryn F (10)
|
FGLLL |
42.27 |
50.81 |
8.54 |
| 14 |
Zaretskaya, Maria (9)
|
FGMSAT |
|
53.20 |
- |
| 15 |
Zapanta, Lana (9)
|
FGLLL |
|
56.98 |
- |
|
Shah, Misha (9)
|
FGWOW |
|
NS |
- |
|
Delos Reyes, Conchessa S (9)
|
FGJDST |
49.61 |
NS |
- |