| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Scott, Macey M (8)
|
NIHAMB |
53.04 |
50.76 |
-2.28 |
| 2 |
Carducci, Elizabeth G (8)
|
NITWST |
51.75 |
50.94 |
-0.81 |
| 3 |
Cook, Hannah R (8)
|
NIHAMB |
59.45 |
56.16 |
-3.29 |
| 4 |
Jankowski, LilaEve L (8)
|
NIGWST |
57.35 |
58.01 |
0.66 |
| 5 |
Ferullo, Abigail C (7)
|
NITWST |
55.71 |
1:01.62 |
5.91 |
| 6 |
Kunz, Amira M (8)
|
NIBCSR |
|
1:02.18 |
- |
| 7 |
Ellison, Paige A (8)
|
NIEMAC |
1:02.39 |
1:07.63 |
5.24 |
| 8 |
Kleinmann, Samantha E (8)
|
NIHAMB |
|
1:08.58 |
- |
| 9 |
Szumigala, Alexis R (8)
|
NIPVSC |
1:05.23 |
1:09.74 |
4.51 |
| 10 |
Yasurek, Rylee (8)
|
NIGWST |
1:20.71 |
1:14.30 |
-6.41 |