| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Conley, Sarah L (14)
|
NITWST |
2:21.10 |
2:22.83 |
1.73 |
| 2 |
Gehm, Kassidy A (14)
|
NIEMAC |
|
2:27.92 |
- |
| 3 |
Choroser, Beatrice C (14)
|
NITWST |
2:27.19 |
2:31.03 |
3.84 |
| 4 |
Jones, Katherine E (13)
|
NITWST |
2:30.96 |
2:33.82 |
2.86 |
| 5 |
Humby, Jessica L (13)
|
NIHAMB |
2:32.47 |
2:34.33 |
1.86 |
| 6 |
Clouser, KaRynn F (14)
|
NIBCSR |
2:43.98 |
2:41.44 |
-2.54 |
| 7 |
Stein, Shannen I (14)
|
NIEMAC |
2:44.02 |
2:41.90 |
-2.12 |
| 8 |
Rozler, Rennee (14)
|
NIHAMB |
2:48.06 |
2:44.58 |
-3.48 |
| 9 |
Musterait, Madeline A (13)
|
NITWST |
2:53.55 |
2:51.41 |
-2.14 |
| 10 |
Malkiewicz, Claire E (13)
|
NITTSC |
|
3:01.04 |
- |
| 11 |
Topolski, Grace E (13)
|
NITTSC |
3:03.43 |
3:01.69 |
-1.74 |
|
Hellwig, Ashleigh R (13)
|
NIHHSC |
|
DQ |
- |