| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Scott, Mia A (10)
|
NIHAMB |
53.72 |
48.35 |
-5.37 |
| 2 |
Wagner, Addison (9)
|
NITWST |
57.82 |
53.06 |
-4.76 |
| 3 |
Seelig, Jordan L (10)
|
NIEAST |
51.77 |
53.60 |
1.83 |
| 4 |
Spino, Kadence E (9)
|
NITWST |
57.89 |
56.12 |
-1.77 |
| 5 |
Hammond, Evelyn R (9)
|
NITWST |
1:00.08 |
56.61 |
-3.47 |
| 6 |
Brackenridge, Peyton Q (10)
|
NITWST |
|
57.08 |
- |
| 7 |
Ellis, Kuepper M (10)
|
NIPVSC |
1:09.08 |
58.01 |
-11.07 |
| 8 |
Oakes, Madelyn G (10)
|
NIHHSC |
56.71 |
59.07 |
2.36 |
| 9 |
Tronetti, Coral L (10)
|
NIEMAC |
1:05.56 |
1:00.92 |
-4.64 |
| 10 |
Schneid, Cassandra M (10)
|
NIHAMB |
|
1:01.33 |
- |
| 11 |
Wilson, Sydney N (9)
|
NIBCSR |
|
1:01.93 |
- |
| 12 |
Sellan, Grace I (9)
|
NITTAC |
1:02.16 |
1:12.02 |
9.86 |
|
Alessi, Willa K (10)
|
NITWST |
|
DQ |
- |
|
Smith, Brooklyn A (9)
|
NIHHSC |
|
DQ |
- |
|
Zent, Madison L (9)
|
NIHAMB |
|
DQ |
- |
|
Mescall, Madigan G (10)
|
NITTAC |
1:05.21 |
DQ |
- |
|
Kirchberger, Addison M (9)
|
NIHAMB |
1:06.75 |
DQ |
- |