| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Lane, Tyler J (14)
|
NITWST |
25.95 |
26.58 |
0.63 |
| 2 |
Sippel, William D (13)
|
NITWST |
27.00 |
26.64 |
-0.36 |
| 3 |
McCrone, Patrick (13)
|
NITWST |
26.96 |
26.79 |
-0.17 |
| 4 |
Donnarummo, Luke C (13)
|
NITWST |
30.29 |
26.91 |
-3.38 |
| 5 |
Coyne, Kiernan P (14)
|
NITWST |
28.50 |
27.84 |
-0.66 |
| 6 |
Wittmeyer, Nicholas R (14)
|
NIHHSC |
28.73 |
29.40 |
0.67 |
| 6 |
Conti, Tyler (13)
|
NITWST |
31.11 |
29.40 |
-1.71 |
| 8 |
Lenz, Reece D (14)
|
NITWST |
31.66 |
31.23 |
-0.43 |
| 9 |
Link, Ayden E (13)
|
NIHAMB |
37.35 |
31.31 |
-6.04 |
| 10 |
Signore, Christopher A (13)
|
NITTSC |
34.73 |
33.76 |
-0.97 |
| 11 |
Sullivan, Jack L (13)
|
NITWST |
37.02 |
37.33 |
0.31 |
| 12 |
Burnett, Jeremiah C (13)
|
NITWST |
34.88 |
45.78 |
10.90 |