| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Noecker, Elle A (12)
|
NITWST |
26.22 |
26.36 |
0.14 |
| 2 |
Kassirer, Natalie G (12)
|
NITWST |
28.81 |
28.63 |
-0.18 |
| 3 |
Kasubowski, Cassie A (11)
|
NIEAST |
32.77 |
31.20 |
-1.57 |
| 4 |
Christopher, Hailey R (12)
|
NIHAMB |
32.50 |
31.75 |
-0.75 |
| 5 |
Ziolkowski, Cosette M (12)
|
NITTAC |
32.88 |
31.95 |
-0.93 |
| 6 |
Sand, Makayla E (12)
|
NITWST |
33.30 |
32.59 |
-0.71 |
| 7 |
Jusiak, Isabella N (12)
|
NIHAMB |
|
33.09 |
- |
| 8 |
Delcamp, Ashlynn R (12)
|
NIHHSC |
32.61 |
33.23 |
0.62 |
| 9 |
Rodgers, Morgan M (12)
|
NITWST |
36.26 |
33.33 |
-2.93 |
| 10 |
Passarell, Charlotte O (12)
|
NITWST |
33.69 |
34.10 |
0.41 |
| 11 |
Kerr, Bell L (11)
|
NIPVSC |
35.56 |
36.15 |
0.59 |
| 12 |
Capozzi, Alivia M (12)
|
NIGWST |
37.40 |
38.33 |
0.93 |
|
Mitchell, Gwenyth E (12)
|
NIEMAC |
29.07 |
NS |
- |
|
Durnford, Riley A (12)
|
NIGWST |
38.27 |
NS |
- |