| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Dunn, Matthew E (12)
|
NIHHSC |
28.91 |
28.87 |
-0.04 |
| 2 |
Marks, Carter A (12)
|
NITWST |
30.86 |
30.60 |
-0.26 |
| 3 |
Bubar, Isaac J (12)
|
NITWST |
34.44 |
30.92 |
-3.52 |
| 4 |
Sippel, Patrick N (12)
|
NITWST |
34.25 |
33.68 |
-0.57 |
| 5 |
Jones, William P (12)
|
NIPVSC |
34.11 |
33.76 |
-0.35 |
| 6 |
Merrill, Christian T (12)
|
NIGWST |
34.21 |
34.13 |
-0.08 |
| 7 |
Bloom, Anthony D (12)
|
NITWST |
34.46 |
34.45 |
-0.01 |
| 8 |
Seifert, Zachary M (12)
|
NITWST |
35.58 |
34.59 |
-0.99 |
| 9 |
Venturo, Aidan J (12)
|
NITWST |
33.19 |
34.65 |
1.46 |
| 10 |
Brauer, Jackson D (12)
|
NITWST |
36.05 |
35.05 |
-1.00 |
| 11 |
Irwin, Cailum P (12)
|
NIHAMB |
37.46 |
36.11 |
-1.35 |
| 12 |
Molina Ayquipa, Santiago I (12)
|
NITWST |
36.99 |
37.64 |
0.65 |
| 13 |
Personius, Hunter J (12)
|
NIHAMB |
|
42.29 |
- |
| 14 |
Thompson, Antoine (12)
|
NIBCSR |
|
45.76 |
- |
| 15 |
Thompson, Antoneo A (12)
|
NIBCSR |
|
45.99 |
- |