| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Peresan, Brody J (8)
|
NIEAST |
42.19 |
43.45 |
1.26 |
| 2 |
Telenko, Vincent M (8)
|
NIGWST |
45.88 |
44.78 |
-1.10 |
| 3 |
Moldenhauer, Thomas C (7)
|
NIEAST |
47.32 |
44.82 |
-2.50 |
| 4 |
Lindquist, Zachary M (8)
|
NIPVSC |
50.61 |
47.14 |
-3.47 |
| 5 |
Askar, Tommy J (8)
|
NIHHSC |
|
51.57 |
- |
| 6 |
Aguglia, Anthony J (7)
|
NITTAC |
58.27 |
52.09 |
-6.18 |
| 7 |
Charlier, Andre D (7)
|
NITTSC |
56.31 |
53.98 |
-2.33 |
| 8 |
Powell, Connor L (8)
|
NITTAC |
48.21 |
55.02 |
6.81 |
| 9 |
Egloff, Rhett S (7)
|
NIEAST |
55.59 |
55.18 |
-0.41 |
| 10 |
Pickard, Aiden A (7)
|
NIHAMB |
|
59.04 |
- |
| 11 |
Waks, Isaac J (6)
|
NIEAST |
1:04.70 |
1:06.45 |
1.75 |
| 12 |
Mihallofski, Adrian G (8)
|
NIHAMB |
1:11.56 |
1:09.47 |
-2.09 |
| 13 |
Schmitt, Nathan J (7)
|
NITTSC |
1:24.85 |
1:20.31 |
-4.54 |