| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Choroser, Beatrice C (15)
|
NITWST |
26.33 |
27.95 |
1.62 |
| 2 |
Nicholl, Daisy K (16)
|
NIEAST |
28.83 |
28.07 |
-0.76 |
| 3 |
Bubar, Carolyn M (15)
|
NITWST |
26.98 |
28.40 |
1.42 |
| 4 |
Polowy, McKayla L (17)
|
NIHHSC |
28.22 |
28.66 |
0.44 |
| 5 |
Yunke, Julia A (15)
|
NITWST |
27.57 |
29.15 |
1.58 |
| 6 |
Stockschlaeder, Amelia R (16)
|
NIHAMB |
28.42 |
29.45 |
1.03 |
| 7 |
Stang, Megan O (17)
|
NIGWST |
29.24 |
30.28 |
1.04 |
| 8 |
Delpriore, Riley A (17)
|
NIGWST |
29.54 |
30.46 |
0.92 |
| 9 |
Ziemba, Sarah J (16)
|
NIHAMB |
30.29 |
30.49 |
0.20 |
| 10 |
Venturo, Madeline T (16)
|
NITWST |
30.13 |
30.95 |
0.82 |
| 11 |
Juzdowski, Alexa H (15)
|
NIHAMB |
30.62 |
31.18 |
0.56 |
| 12 |
DiMartino, Jenna C (15)
|
NIHAMB |
35.56 |
32.39 |
-3.17 |
| 13 |
Baratta, Abby R (15)
|
NIEAST |
32.37 |
32.83 |
0.46 |
| 14 |
Raymond, Gabriella M (16)
|
NIHAMB |
35.49 |
37.70 |
2.21 |