| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Carl, Alexander G (12)
|
NIEMAC |
33.50 |
32.85 |
-0.65 |
| 2 |
Mariotti, Joseph A (12)
|
NIGVST |
32.50 |
33.27 |
0.77 |
| 3 |
Grantier, Payton M (12)
|
NIGVST |
35.96 |
34.50 |
-1.46 |
| 4 |
Merrill, Christian T (11)
|
NIGWST |
34.72 |
36.48 |
1.76 |
| 5 |
LaBenne, Parker L (11)
|
NIGVST |
39.66 |
36.68 |
-2.98 |
| 6 |
Ruszkowski, David J (11)
|
NIEMAC |
38.52 |
37.36 |
-1.16 |
| 7 |
Breton, Dominic C (12)
|
NIFKVL |
37.26 |
39.13 |
1.87 |
| 8 |
Jones, William P (11)
|
NIPVSC |
39.33 |
39.19 |
-0.14 |
| 9 |
Peterson-Volz, Lucas S (11)
|
NIEMAC |
45.44 |
42.83 |
-2.61 |
|
Everett, Ethan R (12)
|
NIGVST |
43.26 |
NS |
- |