| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Wang, Hailey (6)
|
PVRMSC |
55.85 |
45.96 |
-9.89 |
| 2 |
Chen, Livia (6)
|
PVRMSC |
47.64 |
47.40 |
-0.24 |
| 3 |
Diener, Tatiana Q (6)
|
PVRMSC |
50.88 |
52.55 |
1.67 |
| 4 |
Flaim, Isabella J (6)
|
PVRMSC |
|
54.84 |
- |
| 5 |
Wert, Genevieve E (6)
|
PVRMSC |
1:04.28 |
57.28 |
-7.00 |
| 6 |
Taylor, Jovie M (6)
|
PVRMSC |
|
57.84 |
- |
| 7 |
Forsythe, Alexandra K (6)
|
PVFISH |
55.39 |
58.46 |
3.07 |
| 8 |
Song, Evelyn M (6)
|
PVRMSC |
1:11.22 |
59.13 |
-12.09 |
| 9 |
Ngo, Nhi K (6)
|
PVSA |
|
1:00.30 |
- |
| 10 |
Mejia-Mardeeva, Isabel R (6)
|
PVRMSC |
1:02.89 |
1:06.07 |
3.18 |
| 11 |
Cotner Phillips, Lili'u Aloh (6)
|
PVFISH |
|
1:07.05 |
- |
| 12 |
Beinecke, Samantha K (6)
|
PVRMSC |
|
1:08.15 |
- |
| 13 |
Warner, Alyse (5)
|
PVRMSC |
|
1:11.55 |
- |
| 14 |
Yang, Charlotte S (6)
|
PVFISH |
|
1:15.95 |
- |
| 15 |
Johnson, Myloe (4)
|
PVASTS |
|
1:17.04 |
- |
| 16 |
Mahaffy, Caroline A (6)
|
PVFISH |
|
1:23.04 |
- |
| 17 |
Yant, Alanni (5)
|
PVASTS |
1:29.60 |
1:27.46 |
-2.14 |
| 18 |
Sanders, Kayla I (6)
|
PVASTS |
|
1:28.08 |
- |
| 19 |
Moore, Avery J (5)
|
PVASTS |
2:35.04 |
2:02.40 |
-32.64 |
|
Burnayeva, Keira (6)
|
PVRMSC |
|
DQ |
- |
|
Albino-Griffith, Gianna M (5)
|
PVFISH |
1:21.31 |
DQ |
- |