| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
White, Stevarra L (7)
|
PVSA |
40.03 |
40.51 |
0.48 |
| 2 |
Newell, Kiera (7)
|
PVRMSC |
43.94 |
43.21 |
-0.73 |
| 3 |
Payson, Mila C (7)
|
PVRMSC |
|
43.22 |
- |
| 4 |
Banashak, Willa C (7)
|
PVRMSC |
45.75 |
43.42 |
-2.33 |
| 5 |
Mitchell, Isabella L (7)
|
PVRMSC |
47.02 |
46.19 |
-0.83 |
| 6 |
Boone, Elizabeth (7)
|
PVRMSC |
50.09 |
48.48 |
-1.61 |
| 7 |
Cofield, Olivia N (7)
|
PVRMSC |
51.30 |
49.76 |
-1.54 |
| 8 |
Falsone, Alessandra M (7)
|
PVFISH |
46.00 |
50.61 |
4.61 |
| 9 |
Sun, Joanna (7)
|
PVRMSC |
54.88 |
51.86 |
-3.02 |
| 10 |
Lei, Catherine N (7)
|
PVSA |
56.69 |
53.98 |
-2.71 |
| 11 |
Zhou, Claire (7)
|
PVRMSC |
52.66 |
54.71 |
2.05 |
| 12 |
Erwin, Caroline N (7)
|
PVFISH |
1:09.10 |
57.17 |
-11.93 |
| 13 |
Brooke, Victoria R (7)
|
PVRMSC |
|
59.20 |
- |
| 14 |
Liu, Journey (7)
|
PVRMSC |
53.08 |
1:00.67 |
7.59 |
| 15 |
Wang, Brianna (7)
|
PVRMSC |
59.81 |
1:04.44 |
4.63 |
|
Hu, Sophia (7)
|
PVRMSC |
|
DQ |
- |
|
Munkhbat, Saruul (7)
|
PVRMSC |
|
DQ |
- |
|
Pierouchakos, Alexandra G (7)
|
PVBWST |
|
DQ |
- |
|
Yu, Marigold (7)
|
PVFISH |
1:01.02 |
DQ |
- |