| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Iverson, Amanda M (17)
|
WILAXY |
55.15 |
57.18 |
2.03 |
| 2 |
Onsager, Skylar M (15)
|
WIMYST |
56.61 |
57.27 |
0.66 |
| 3 |
Adams, Rebecca L (17)
|
WICVY |
58.01 |
58.04 |
0.03 |
| 4 |
Nguyen, Amber T (17)
|
WILAXY |
1:00.06 |
58.41 |
-1.65 |
| 5 |
Lettner, Elle (16)
|
MNWIN |
58.56 |
58.91 |
0.35 |
| 6 |
Ernst, Kyler (16)
|
MNWIN |
59.17 |
59.34 |
0.17 |
| 7 |
Czerwonka, Sophie E (17)
|
WISPDY |
1:00.12 |
1:00.03 |
-0.09 |
| 8 |
Pike, Ava (15)
|
MNWIN |
1:02.28 |
1:01.24 |
-1.04 |
| 9 |
Stimac, Paige E (15)
|
WISWC |
1:03.77 |
1:03.36 |
-0.41 |
| 10 |
Kay-Tsumagari, Erika M (17)
|
WILAXY |
1:01.66 |
1:04.10 |
2.44 |
| 11 |
Johnston, Anna E (15)
|
WISPDY |
1:02.51 |
1:04.93 |
2.42 |
| 12 |
Wickstrom, Ava (15)
|
MNWIN |
1:05.55 |
1:05.35 |
-0.20 |
| 13 |
Archibald-Smith, Madalyn C (17)
|
WIECY |
1:04.26 |
1:05.79 |
1.53 |
| 14 |
White, Alexandra J (16)
|
WILAXY |
1:02.34 |
1:06.60 |
4.26 |
|
Tichy, Saige (18)
|
WIMYST |
1:00.51 |
NS |
- |