| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Carlos, Nathanial J (16)
|
NIMETS |
22.14 |
22.86 |
0.72 |
| 2 |
Rourke, Conlan J (16)
|
NISYCH |
23.74 |
23.74 |
0.00 |
| 3 |
Tarolli, Vincent M (15)
|
NICSCI |
23.62 |
23.84 |
0.22 |
| 4 |
Shelmidine, Nolan E (16)
|
NIMETS |
25.32 |
25.32 |
0.00 |
| 5 |
Busch, Nolan S (17)
|
NIOLSC |
24.77 |
25.89 |
1.12 |
| 6 |
Burke, Connor D (13)
|
NISYCH |
26.47 |
26.17 |
-0.30 |
| 7 |
Alexander, Nathan R (13)
|
NIJETS |
27.12 |
26.79 |
-0.33 |
| 8 |
Diel, Robert M (15)
|
NISYCH |
27.11 |
26.80 |
-0.31 |
| 9 |
Van Kersbergen, Gavin M (11)
|
NISKAN |
27.13 |
26.87 |
-0.26 |
| 10 |
Ignacio, Emmanuel T (15)
|
NIOLSC |
27.67 |
26.99 |
-0.68 |
| 11 |
Williams, Jonah F (12)
|
NISKAN |
26.91 |
27.05 |
0.14 |
| 12 |
Denkenberger, Quinten M (13)
|
NIOLSC |
27.90 |
28.31 |
0.41 |
| 13 |
Miller, Treyse B (12)
|
NIMETS |
29.87 |
29.24 |
-0.63 |
| 14 |
Walz, Nathan A (13)
|
NISYCH |
30.46 |
30.15 |
-0.31 |
| 15 |
Finnerty, Sean P (13)
|
NICSCI |
32.29 |
30.42 |
-1.87 |
| 16 |
Hatch, Collin M (13)
|
NIMETS |
33.57 |
31.64 |
-1.93 |
| 17 |
Newsome, George W (11)
|
NIPMSC |
32.55 |
31.70 |
-0.85 |
| 18 |
Loomis, Jesse J (13)
|
NIMETS |
36.06 |
35.58 |
-0.48 |
| 19 |
Hatch, Lincoln E (11)
|
NIMETS |
|
50.93 |
- |
|
Clark, Ethan D (11)
|
NIMETS |
33.24 |
NS |
- |