| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Messaris, Charlotte R (16)
|
MDEST |
32.32 |
32.83 |
0.51 |
| 2 |
Claycomb, Julie A (16)
|
MDBAAC |
|
33.95 |
- |
| 3 |
Skvortsova, Margarita (15)
|
MDEST |
34.66 |
34.03 |
-0.63 |
| 4 |
Hines, Nyah E (17)
|
MDEST |
34.80 |
34.58 |
-0.22 |
| 5 |
Brannon, Abigail E (16)
|
MDACSS |
35.40 |
34.78 |
-0.62 |
| 6 |
Meyer, Emily T (15)
|
MDACSS |
35.73 |
35.62 |
-0.11 |
| 7 |
Picker, Zoe E (15)
|
MDEST |
31.59 |
35.93 |
4.34 |
| 8 |
Wright, Alexa J (15)
|
MDACSS |
|
36.63 |
- |
| 9 |
Anderson, Kayla A (15)
|
MDACSS |
38.04 |
38.31 |
0.27 |
| 10 |
Woldeselassie, Elizabeth D (15)
|
MDEST |
39.58 |
40.03 |
0.45 |
| 11 |
Powell, Lillian R (15)
|
MDACSS |
45.17 |
41.14 |
-4.03 |
| 12 |
Amato, Anna B (15)
|
MDBAAC |
42.33 |
42.11 |
-0.22 |