| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Chhun, Austin (8)
|
MDBAAC |
44.85 |
45.40 |
0.55 |
| 2 |
McCallister, Prince K (8)
|
MDMAS |
48.18 |
47.94 |
-0.24 |
| 3 |
Ohtani, Haruki (8)
|
MDCAA |
48.43 |
48.61 |
0.18 |
| 4 |
Chiang, Isaac I (8)
|
MDCAA |
50.55 |
50.26 |
-0.29 |
| 5 |
Killebrew, Jai F (8)
|
MDASC |
51.59 |
50.54 |
-1.05 |
| 6 |
Zaidi, Albert Z (8)
|
MDCAC |
53.60 |
52.87 |
-0.73 |
| 7 |
Rigney, Aidan T (8)
|
MDCBAC |
50.77 |
52.90 |
2.13 |
| 8 |
Frank, Leo G (8)
|
MDNBAC |
55.78 |
55.10 |
-0.68 |
| 9 |
Vijayan, Jackson O (8)
|
MDMSC |
58.12 |
55.13 |
-2.99 |
| 10 |
Wirth, Micah C (8)
|
MDFAST |
58.01 |
55.58 |
-2.43 |
| 11 |
Bowman, James F (8)
|
MDACA |
1:04.51 |
55.60 |
-8.91 |
| 12 |
Winters, Kyle D (8)
|
MDSPRC |
56.91 |
55.65 |
-1.26 |
| 13 |
Gloss, John R (8)
|
MDMAC |
58.90 |
55.91 |
-2.99 |
| 14 |
Meigel, Elias M (8)
|
MDASC |
59.02 |
56.48 |
-2.54 |
| 15 |
Morrison, Richard S (8)
|
MDASC |
58.30 |
57.01 |
-1.29 |
| 16 |
De Los Reyes-Kundey, Aden A (8)
|
MDMAC |
57.66 |
57.15 |
-0.51 |
| 17 |
O'Shea, Matthew T (8)
|
MDHOCO |
55.72 |
57.39 |
1.67 |
| 18 |
Hilotii, Bogdan (8)
|
MDCAA |
1:00.56 |
58.47 |
-2.09 |
| 19 |
Dolezel, Erik W (8)
|
MDCAA |
58.08 |
58.95 |
0.87 |
| 20 |
Ahn, Ryan Y (8)
|
MDCAA |
1:00.85 |
59.01 |
-1.84 |
| 21 |
Chand, Shaurya A (8)
|
MDCAA |
53.78 |
59.61 |
5.83 |
| 22 |
Holt, Dylan T (8)
|
MDFSC |
59.41 |
1:00.11 |
0.70 |
| 23 |
Malhotra, Ronav (8)
|
MDMAS |
|
1:00.53 |
- |
| 24 |
Zhao, Justin X (8)
|
MDCAA |
59.39 |
1:00.80 |
1.41 |
| 25 |
Chen, Boyce (8)
|
MDCAA |
1:00.30 |
1:01.36 |
1.06 |
| 26 |
Weaver, Landen J (8)
|
MDACA |
59.81 |
1:04.00 |
4.19 |
| 27 |
Tokarsky, Gabriel E (8)
|
MDHOCO |
1:04.07 |
1:04.87 |
0.80 |
| 28 |
Vicinelly, Anthony J (8)
|
MDFSC |
1:03.25 |
1:05.54 |
2.29 |
| 29 |
Liang, Derek C (8)
|
MDMAC |
1:11.34 |
1:06.84 |
-4.50 |
| 30 |
Parker, John L (8)
|
MDOPST |
1:15.10 |
1:10.80 |
-4.30 |
| 31 |
Zumbrunn, Zachary D (8)
|
MDFAST |
|
1:11.80 |
- |
| 32 |
Su, Leo (8)
|
MDRAC |
1:16.43 |
1:15.08 |
-1.35 |
| 33 |
Pearson, Levi E (8)
|
MDROCK |
|
1:23.18 |
- |
| 34 |
Yankovic, Julian O (8)
|
MDMAS |
1:25.62 |
1:23.20 |
-2.42 |
| 35 |
Liang, Daniel C (8)
|
MDMAC |
|
1:35.49 |
- |
| 36 |
Mozzo, Alexander K (8)
|
MDCBAC |
1:29.12 |
1:38.66 |
9.54 |
| 37 |
Mudd, Brandon C (8)
|
MDCAC |
1:19.69 |
2:17.60 |
57.91 |
|
Matsumoto, Wataru N (8)
|
MDJCC |
|
DQ |
- |
|
Wang, Andrew (8)
|
MDCAA |
|
DQ |
- |
|
Meade, Aiden R (8)
|
MDASC |
|
DQ |
- |
|
Fisher, Bentley S (8)
|
MDMAC |
|
NS |
- |
|
Hogue, Ryan J (8)
|
MDRAC |
54.62 |
NS |
- |