| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Gomez, Isabelle (16)
|
OHLAKY |
2:24.74 |
2:29.06 |
4.32 |
| 2 |
McMenemy, Kelsey L (17)
|
OHPCY |
2:23.67 |
2:33.12 |
9.45 |
| 3 |
Johnson, Alexandra F (16)
|
OHABLY |
2:24.04 |
2:33.87 |
9.83 |
| 4 |
O'Toole, Margaret M (15)
|
OHABLY |
2:30.59 |
2:33.95 |
3.36 |
| 5 |
Kamstra, Brooke E (17)
|
OHPCY |
2:33.49 |
2:42.49 |
9.00 |
| 6 |
Knapek, Kaitlin N (16)
|
OHLAKY |
2:42.88 |
2:43.36 |
0.48 |
| 7 |
Kuertz, Maria E (16)
|
OHGMVY |
|
2:43.88 |
- |
| 8 |
Koetter, Elizabeth S (16)
|
OHBASH |
2:43.20 |
2:45.03 |
1.83 |
| 9 |
Roberts, Lily A (15)
|
OHABLY |
|
2:45.64 |
- |
| 10 |
Marini, Sofia A (17)
|
OHLAKY |
|
2:46.52 |
- |
| 11 |
Zhao, Kathy J (17)
|
OHBASH |
2:54.09 |
2:46.71 |
-7.38 |
| 12 |
Hoffman, Ashlyn M (15)
|
OHBASH |
2:43.26 |
2:46.85 |
3.59 |
| 13 |
Pivor, Isabella R (17)
|
OHBASH |
|
2:48.17 |
- |
| 14 |
Hubbell, Kendall P (16)
|
OHBASH |
2:41.16 |
2:48.48 |
7.32 |
| 15 |
Johnson, Lucia M (16)
|
OHABLY |
|
2:48.64 |
- |
| 16 |
Cowan, Lily (17)
|
OHABLY |
2:44.21 |
2:49.10 |
4.89 |
| 17 |
Getter, Courtney E (16)
|
OHKEY |
|
2:49.36 |
- |
| 18 |
Haas, Abigail E (15)
|
OHKEY |
2:49.86 |
2:49.44 |
-0.42 |
| 19 |
Wilson, Olivia M (15)
|
OHABLY |
2:41.49 |
2:50.39 |
8.90 |
| 20 |
Johnson, Gianna C (16)
|
OHAMFY |
|
2:54.08 |
- |
| 21 |
Trokhan, Case T (17)
|
OHGMVY |
|
2:54.57 |
- |
| 22 |
Rice, Lily A (17)
|
OHRCDB |
3:05.50 |
2:55.14 |
-10.36 |
| 23 |
Woolf-Isbel, MacKenzie K (16)
|
OHKEY |
3:00.66 |
3:03.44 |
2.78 |
| 24 |
Sickmeier, Maria T (16)
|
OHRCDB |
|
3:04.94 |
- |
| 25 |
Chesson, Victoria M (17)
|
OHPCY |
|
3:05.26 |
- |
| 26 |
Wilking, Emma A (15)
|
OHPCY |
|
3:12.86 |
- |
| 27 |
Bhatti, Angelica (15)
|
OHLAKY |
|
3:16.30 |
- |
|
Hoffmann, Grace E (16)
|
OHPCY |
|
DQ |
- |
|
Sunderland, McKenna M (16)
|
OHBASH |
2:42.03 |
NS |
- |
|
Wentzel, Hannah R (15)
|
OHKEY |
3:01.24 |
NS |
- |