| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Cochran, Jeffrey C (14)
|
LARRAC |
29.27 |
28.54 |
-0.73 |
| 2 |
Menchhofer, Bryce R (13)
|
OHBASH |
31.01 |
30.09 |
-0.92 |
| 3 |
Lamping, Adam J (14)
|
OHPCY |
30.41 |
31.29 |
0.88 |
| 4 |
Kairschner, Jackson M (14)
|
LARRAC |
31.77 |
31.37 |
-0.40 |
| 5 |
Huggins, Sean C (14)
|
OHBASH |
31.08 |
31.62 |
0.54 |
| 6 |
Held, Drew G (14)
|
OHBASH |
34.40 |
32.53 |
-1.87 |
| 7 |
Koenigsknecht, Benett D (14)
|
OHRCDB |
|
33.02 |
- |
| 8 |
Roberts, Cameron L (14)
|
OHKEY |
33.55 |
33.24 |
-0.31 |
| 9 |
Fyffe, Chase T (13)
|
OHSPY |
35.59 |
33.77 |
-1.82 |
| 10 |
Yeager, Samuel M (14)
|
MIRAYS |
|
34.11 |
- |
| 11 |
McClain, Marcus (13)
|
ZZAMFY |
40.24 |
34.62 |
-5.62 |
| 12 |
Kiihnl, Christopher A (13)
|
LARRAC |
40.81 |
35.83 |
-4.98 |
| 13 |
Platt, Ryoma T (13)
|
OHABLY |
38.85 |
35.86 |
-2.99 |
| 14 |
Gruber, Christian W (13)
|
OHLAKY |
40.75 |
36.08 |
-4.67 |
| 15 |
Bogen, Joshua D (13)
|
OHPCY |
36.13 |
36.53 |
0.40 |
| 16 |
Smith, Colin T (13)
|
OHKEY |
41.34 |
37.62 |
-3.72 |
| 17 |
Tullis, Noah C (13)
|
OHMCY |
|
40.74 |
- |
| 18 |
Mason, Benjamin J (13)
|
OHCFY |
|
45.28 |
- |
| 19 |
Womble, Alexander (13)
|
OHHHY |
|
57.62 |
- |
|
Clark, Nathaniel G (14)
|
OHPCY |
|
NS |
- |