| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Kyre, Emily T (14)
|
OHKEY |
2:41.42 |
2:40.55 |
-0.87 |
| 2 |
Johnson, McKenna A (13)
|
OHABLY |
2:45.26 |
2:44.48 |
-0.78 |
| 3 |
Ryan, Kate M (14)
|
OHBASH |
2:59.01 |
2:45.80 |
-13.21 |
| 4 |
Burke, Kathleen M (13)
|
OHPCY |
3:03.60 |
2:48.45 |
-15.15 |
| 5 |
Molina, Sophia L (13)
|
OHLAKY |
3:10.66 |
2:48.72 |
-21.94 |
| 6 |
Simon, Paige E (14)
|
OHKEY |
|
2:55.67 |
- |
| 7 |
Schneider, Victoria C (14)
|
OHLAKY |
|
2:57.29 |
- |
| 8 |
Roberts, Anna E (13)
|
OHABLY |
|
3:00.43 |
- |
| 9 |
Schroeder, Isabelle G (13)
|
OHBASH |
|
3:03.36 |
- |
| 10 |
Adams, Laine C (14)
|
OHABLY |
3:12.91 |
3:04.12 |
-8.79 |
| 11 |
Shaffer, Alyssa N (13)
|
OHSPY |
3:09.91 |
3:05.09 |
-4.82 |
| 12 |
Quinn, Delaney A (14)
|
OHBASH |
3:20.22 |
3:05.40 |
-14.82 |
| 13 |
Kutter, Abigail T (13)
|
OHKEY |
3:21.85 |
3:08.09 |
-13.76 |
| 14 |
Smith, Madison E (13)
|
OHKEY |
|
3:11.10 |
- |
| 15 |
Hubbell, Keally P (13)
|
OHBASH |
3:18.71 |
3:11.81 |
-6.90 |
| 16 |
Revelee, Layla M (13)
|
OHGMVY |
3:34.67 |
3:11.91 |
-22.76 |
| 17 |
Auger, Annabel M (13)
|
OHPCY |
3:31.99 |
3:15.63 |
-16.36 |
| 18 |
Homan, Lucy R (13)
|
OHAMFY |
|
3:17.20 |
- |
| 19 |
Thomas, Averie J (14)
|
OHPCY |
|
3:17.75 |
- |
| 20 |
Nelson, Penelope A (13)
|
OHKEY |
|
3:23.79 |
- |
| 21 |
Cabrera, Solvey A (13)
|
OHGMVY |
|
3:36.37 |
- |
| 22 |
Ashburn, Jalee D (13)
|
KYNLY |
|
3:52.06 |
- |
|
Sutphin, Isabella L (14)
|
OHABLY |
2:49.31 |
NS |
- |
|
Glover, Jazzyona L (13)
|
OHPCY |
|
NS |
- |
|
Caplinger, Katie L (13)
|
OHHHY |
3:34.93 |
NS |
- |
|
LeRoy, Catherine M (14)
|
OHBASH |
3:13.33 |
NS |
- |
|
Tenhundfeld, Emma L (14)
|
OHKEY |
2:35.98 |
NS |
- |
|
Derr, Penelope F (13)
|
OHSPY |
3:00.68 |
NS |
- |