| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Yordi, Joshua (10)
|
FGOSC |
39.42 |
37.41 |
-2.01 |
| 2 |
Viazmetinov, Oleksii (9)
|
FGSOFL |
39.58 |
38.57 |
-1.01 |
| 3 |
Fothergill, Liam (9)
|
FGMWA |
39.62 |
39.04 |
-0.58 |
| 4 |
Gerasim, George (8)
|
FGNMST |
41.67 |
39.05 |
-2.62 |
| 5 |
Iodice, Diego D (10)
|
FGCM |
41.09 |
39.08 |
-2.01 |
| 6 |
Kotz-Agramonte, Jake (10)
|
FGCM |
40.72 |
39.43 |
-1.29 |
| 7 |
Adan, Daniel S (9)
|
FGMACM |
40.74 |
39.66 |
-1.08 |
| 8 |
Lieberman, Oliver J (10)
|
FGMBS |
39.88 |
39.91 |
0.03 |
| 9 |
Fernandez, Luca V (10)
|
FGEA |
41.08 |
39.93 |
-1.15 |
| 10 |
Harries, Ryan T (9)
|
FGSOFL |
41.40 |
40.51 |
-0.89 |
| 11 |
Meltzer, Russell A (10)
|
FGMCDA |
41.76 |
40.65 |
-1.11 |
| 12 |
Kokidko, Nicolas N (10)
|
FGGRSC |
42.38 |
41.30 |
-1.08 |
| 13 |
Pedraja, William G (10)
|
FGCANE |
49.01 |
41.43 |
-7.58 |
| 14 |
Infante, Alejandro R (10)
|
FGCANE |
41.90 |
41.95 |
0.05 |
| 15 |
Negrin, Axel (9)
|
FGUN-S |
40.52 |
42.00 |
1.48 |
| 16 |
Fanjul, Benjamin G (10)
|
FGCANE |
41.40 |
42.71 |
1.31 |
|
Villar, Ilan W (9)
|
FGCANE |
49.01 |
NS |
- |
|
Rodriguez, Marco A (10)
|
FGBMHG |
40.64 |
NS |
- |
|
Naranjo, Robinson (9)
|
FGBMHG |
39.94 |
NS |
- |
|
Gonzalez, Enzo (10)
|
FGBMHG |
41.77 |
NS |
- |
|
Fernandez, Aaron O (9)
|
FGBMHG |
41.74 |
NS |
- |