| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Cave, Mallory C (12)
|
NCPAC |
34.94 |
34.30 |
-0.64 |
| 2 |
Kiser, Ava C (11)
|
NCPAC |
35.42 |
34.70 |
-0.72 |
| 3 |
Caro, Ilihanie (12)
|
NCPAC |
40.60 |
38.48 |
-2.12 |
| 4 |
Collins, Kaci B (12)
|
NCPAC |
37.63 |
38.98 |
1.35 |
| 5 |
Soriano, Cierra J (12)
|
NCPAC |
39.97 |
40.03 |
0.06 |
| 6 |
Vargas Burgos, Liset (11)
|
NCPAC |
51.67 |
40.71 |
-10.96 |
| 7 |
Grabs, Gordon M (12)
|
NCPAC |
|
43.19 |
- |
| 8 |
Pennell, Kate M (11)
|
NCNSS |
46.84 |
44.47 |
-2.37 |
| 9 |
Poindexter, Reagan Q (12)
|
NCPAC |
47.79 |
48.58 |
0.79 |
| 10 |
Brantley, Chloe M (11)
|
NCPAC |
|
48.62 |
- |
| 11 |
Haines, Aniela C (12)
|
NCNSS |
50.36 |
49.05 |
-1.31 |
| 12 |
Ring, Brylee D (11)
|
NCPAC |
|
55.54 |
- |
| 13 |
Mendez, Ariel A (11)
|
NCPAC |
|
56.31 |
- |
| 14 |
Hemans, Mackenzie C (11)
|
NCQCD |
54.00 |
58.07 |
4.07 |
|
Smith, Brenn M (11)
|
NCBAD |
|
DQ |
- |