| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Rassiane, Aliya C (10)
|
AMMMSA |
3:08.05 |
2:50.14 |
-17.91 |
| 2 |
Ruperto, Emily (10)
|
AMGPAC |
3:11.58 |
2:56.49 |
-15.09 |
| 3 |
Daugherty, Amara Q (10)
|
AMANSC |
2:59.92 |
2:57.20 |
-2.72 |
| 4 |
Daugherty, Lillian N (10)
|
AMANSC |
2:55.17 |
2:57.88 |
2.71 |
| 5 |
Jochims, Ava G (10)
|
AMRXA |
3:05.36 |
2:59.36 |
-6.00 |
| 6 |
Biltz, Avery M (10)
|
AMCSC |
3:22.31 |
3:10.45 |
-11.86 |
| 7 |
Calhoun, Whitney J (9)
|
AMRXA |
3:30.99 |
3:17.77 |
-13.22 |
| 8 |
Fisher, Carrie J (10)
|
AMSFAC |
3:33.19 |
3:22.10 |
-11.09 |
| 9 |
Bauer, Mackenzie G (10)
|
AMPTSC |
3:34.38 |
3:22.38 |
-12.00 |
| 10 |
Zakrzewski, Brinley C (10)
|
AMWAAC |
3:30.62 |
3:26.00 |
-4.62 |