| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Pratt, Riley M (14)
|
AMKW |
3:14.04 |
3:12.11 |
-1.93 |
| 2 |
Bliss, Evelyn (14)
|
AMUN-R |
3:19.47 |
3:16.59 |
-2.88 |
| 3 |
Walko, Julia F (13)
|
AMNAC |
3:22.65 |
3:16.71 |
-5.94 |
| 4 |
Wen, Gloria Y (13)
|
AMKW |
3:27.15 |
3:18.75 |
-8.40 |
| 5 |
Geary, Sydney M (13)
|
AMSFAC |
3:19.77 |
3:23.53 |
3.76 |
| 6 |
Kling, Isabella R (13)
|
AMSVSC |
3:32.17 |
3:24.50 |
-7.67 |
| 7 |
DeVito, Mia (13)
|
AMCSC |
3:28.01 |
3:25.63 |
-2.38 |
| 8 |
Haas, Katherine R (13)
|
AMKW |
3:27.55 |
3:26.58 |
-0.97 |
| 9 |
Holmes, Morgan S (14)
|
AMMMSA |
2:50.12 |
3:28.42 |
38.30 |
| 10 |
Strotmeyer, Kalina S (13)
|
AMMLAC |
3:38.15 |
3:29.34 |
-8.81 |
| 11 |
Richards, Abbie N (13)
|
AMTRIN |
2:59.64 |
3:31.88 |
32.24 |
| 12 |
LaRoche, Francesca L (13)
|
AMWHAT |
3:24.72 |
3:36.36 |
11.64 |
| 13 |
Wilson, Taryn R (13)
|
AMSVSC |
3:10.67 |
3:42.58 |
31.91 |
| 14 |
Dunn, Riley N (14)
|
AMTRIN |
3:40.53 |
3:44.22 |
3.69 |