| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Raman, Alessia M (9)
|
AMKW |
3:15.77 |
3:06.38 |
-9.39 |
| 2 |
McKiernan, Morgan E (10)
|
AMBPR |
3:39.17 |
3:09.19 |
-29.98 |
| 3 |
Malacki, Paisley (10)
|
AMSPAC |
3:30.00 |
3:10.26 |
-19.74 |
| 4 |
Chapman, Avery J (10)
|
AMMLAC |
3:54.63 |
3:19.70 |
-34.93 |
| 5 |
Stoecklein, Caroline M (10)
|
AMSFAC |
3:43.19 |
3:21.98 |
-21.21 |
| 6 |
Fisher, Carrie J (10)
|
AMSFAC |
4:00.99 |
3:30.22 |
-30.77 |
| 7 |
Munoz, Kimberly A (10)
|
AMSFAC |
3:52.19 |
3:31.63 |
-20.56 |
| 8 |
Zakrzewski, Brinley C (10)
|
AMWAAC |
3:33.53 |
3:32.27 |
-1.26 |
| 9 |
Clark, Makenzie E (10)
|
AMSVSC |
4:26.08 |
3:39.50 |
-46.58 |
| 10 |
Josephson, Mara A (10)
|
AMSVSC |
3:48.74 |
3:42.56 |
-6.18 |
| 11 |
McCarthy, Morgan M (10)
|
AMWAAC |
3:37.19 |
3:43.06 |
5.87 |
| 12 |
Schnur, Abigail R (10)
|
AMSVSC |
4:05.41 |
3:46.01 |
-19.40 |
| 13 |
Golomb, Ella L (10)
|
AMKW |
4:01.02 |
3:50.93 |
-10.09 |
|
Datt, Elizabeth L (10)
|
AMKW |
3:26.78 |
DQ |
- |
|
Osmon, Symrin L (9)
|
AMSFAC |
4:24.19 |
DQ |
- |
|
Chilenski, Cayden J (10)
|
AMPEAQ |
3:47.36 |
DQ |
- |