| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Young, Kevin G (10)
|
AMGCY |
41.33 |
44.44 |
3.11 |
| 2 |
Burnworth, Aidan A (10)
|
AMSVSC |
45.58 |
44.60 |
-0.98 |
| 3 |
Lozowski, Nicholas R (9)
|
AMSVSC |
51.09 |
46.90 |
-4.19 |
| 4 |
Marcincavage, Roddy G (10)
|
AMNAC |
49.54 |
47.36 |
-2.18 |
| 5 |
Powell, Caden R (10)
|
AMBPR |
54.63 |
47.71 |
-6.92 |
| 6 |
Book, Parker R (10)
|
AMSRST |
46.89 |
48.76 |
1.87 |
| 7 |
Petrina, William R (9)
|
AMNGAC |
47.82 |
48.94 |
1.12 |
| 8 |
Lash, Liam (8)
|
AMUN-R |
51.22 |
49.21 |
-2.01 |
| 9 |
McKelvey, Greyson O (9)
|
AMNGAC |
53.74 |
49.60 |
-4.14 |
| 10 |
Mihalovich, Alexander C (10)
|
AMPTSC |
52.52 |
49.82 |
-2.70 |
| 11 |
Turner, Joshua O (9)
|
AMRACE |
55.73 |
50.56 |
-5.17 |
| 12 |
Pickett, Noah M (10)
|
AMMAC |
55.01 |
51.02 |
-3.99 |
| 13 |
Rubinosky, Braden M (10)
|
AMSVSC |
54.43 |
51.66 |
-2.77 |
| 14 |
Kvocak, Benjamin S (10)
|
AMFAST |
52.99 |
52.50 |
-0.49 |
| 15 |
Tucker, Jacob A (9)
|
AMCSC |
54.56 |
52.53 |
-2.03 |
| 16 |
Ruff, Desmond C (10)
|
AMKW |
1:00.62 |
53.23 |
-7.39 |
| 17 |
Filer, Evan M (9)
|
AMPTSC |
1:09.19 |
55.13 |
-14.06 |
| 18 |
Ruggeri, Peter J (10)
|
AMRACE |
1:06.00 |
56.58 |
-9.42 |
| 19 |
Buzzard, Simon J (7)
|
AMNGAC |
58.19 |
58.50 |
0.31 |
| 20 |
Pickett, Christopher B (8)
|
AMMAC |
1:00.11 |
1:00.64 |
0.53 |
| 21 |
Chiodo, Cameron M (8)
|
AMPTSC |
55.19 |
1:00.67 |
5.48 |
| 22 |
McKinney, Jacoby E (9)
|
AMRACE |
1:01.00 |
1:01.99 |
0.99 |
| 23 |
Smith, Aiden J (9)
|
AMCSC |
58.68 |
1:02.09 |
3.41 |
| 24 |
Haas, William C (8)
|
AMKW |
1:05.02 |
1:03.36 |
-1.66 |
| 25 |
Hall, Jacob M (8)
|
AMRACE |
1:13.00 |
1:12.04 |
-0.96 |
| 26 |
Singh, Sahib (8)
|
AMGPAC |
1:44.11 |
1:49.46 |
5.35 |
|
Strock, Thomas J (9)
|
AMBPR |
52.13 |
DQ |
- |
|
Cohen, Parker T (10)
|
AMFAST |
1:08.99 |
NS |
- |