| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Mittleider, Lauren A (11)
|
AMPEAQ |
2:33.49 |
2:34.14 |
0.65 |
| 2 |
Siegel, Thea E (11)
|
AMURXA |
2:32.70 |
2:34.86 |
2.16 |
| 3 |
MacAdams, Ainsley L (11)
|
AMPEAQ |
2:37.39 |
2:36.87 |
-0.52 |
| 4 |
Kass, Addison A (11)
|
AMRXA |
2:37.97 |
2:38.97 |
1.00 |
| 5 |
Smith, Rachel M (11)
|
AMBYAC |
2:54.39 |
2:56.88 |
2.49 |
| 6 |
Augustynowicz, Macie A (11)
|
AMPTSC |
2:58.93 |
2:58.62 |
-0.31 |
| 7 |
Prindle, Casey S (11)
|
AMBPR |
3:18.39 |
3:12.51 |
-5.88 |
| 8 |
Hudak, Chelsea M (11)
|
AMBPR |
3:44.17 |
3:18.49 |
-25.68 |
| 9 |
Polinski, Anna S (11)
|
AMMLAC |
3:25.02 |
3:22.32 |
-2.70 |
| 10 |
Stoecklein, Caroline M (11)
|
AMSFAC |
3:19.48 |
3:23.98 |
4.50 |
| 11 |
Ginsburg, Lauren E (11)
|
AMPEAQ |
3:22.45 |
3:25.56 |
3.11 |
| 12 |
Nicastro, Tomasina D (11)
|
AMWAAC |
3:29.69 |
3:27.23 |
-2.46 |
| 13 |
Clodfelter, Claire L (11)
|
AMPEAQ |
3:14.29 |
3:33.53 |
19.24 |
| 14 |
Alston, Lillyanna R (11)
|
AMBPR |
4:00.13 |
3:33.88 |
-26.25 |