| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Muzzy, Ava M (12)
|
VAGATR |
4:46.18 |
4:41.84 |
-4.34 |
| 2 |
Shao, Sophie W (12)
|
AMKW |
5:11.26 |
4:46.84 |
-24.42 |
| 3 |
Li, Vivien Y (12)
|
AMANSC |
5:05.13 |
4:57.16 |
-7.97 |
| 4 |
Davis, Grey T (11)
|
VAGATR |
5:04.17 |
4:57.52 |
-6.65 |
| 5 |
Eiben, Natalie J (12)
|
AMFAST |
5:07.97 |
5:01.85 |
-6.12 |
| 6 |
Fourie, Trinity C (12)
|
MRCOND |
5:23.95 |
5:09.45 |
-14.50 |
| 7 |
Gennarelli, Maichina E (11)
|
MRCOND |
5:13.44 |
5:10.61 |
-2.83 |
| 8 |
Blakley, Paityn S (12)
|
AMFAST |
5:19.69 |
5:14.82 |
-4.87 |
| 9 |
Ma, Sophia (12)
|
VAGATR |
5:25.73 |
5:15.92 |
-9.81 |
| 10 |
Arciniegas, Laura (11)
|
MRCOND |
5:19.77 |
5:18.25 |
-1.52 |
| 11 |
Kern, Sarah J (12)
|
MAGPAC |
5:20.22 |
5:18.84 |
-1.38 |
| 12 |
Little, Anna R (12)
|
AMNAC |
5:17.42 |
5:20.29 |
2.87 |
| 13 |
Miller, Eliza J (11)
|
AMRXA |
6:13.42 |
5:28.06 |
-45.36 |
| 14 |
Gallingane, Sophia L (12)
|
AMSVSC |
5:29.20 |
5:30.88 |
1.68 |
|
Whitlow, Lane O (12)
|
VAGATR |
5:07.17 |
DQ |
- |