| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Turner, Ryan T (14)
|
AMRACE |
51.62 |
52.02 |
0.40 |
| 2 |
Tudorascu, Andrei (14)
|
AMMLAC |
53.44 |
52.45 |
-0.99 |
| 3 |
Shipley, Maxwell J (14)
|
AMPEAQ |
55.86 |
56.25 |
0.39 |
| 4 |
McClellan, Sean G (14)
|
AMURXA |
1:12.85 |
57.13 |
-15.72 |
| 5 |
Le, Kenneth B (14)
|
AMMLAC |
57.81 |
57.26 |
-0.55 |
| 6 |
Klepchick, Matthew P (14)
|
AMMLAC |
59.11 |
57.53 |
-1.58 |
| 7 |
Petkov, Alexander M (14)
|
AMPEAQ |
59.27 |
57.91 |
-1.36 |
| 8 |
Manelis, Peter A (14)
|
AMRXA |
58.59 |
58.32 |
-0.27 |
| 9 |
Jacobs-Alarcon, Nicolas A (14)
|
AMURXA |
58.97 |
59.07 |
0.10 |
| 10 |
Jones, Asher R (14)
|
AMURXA |
1:08.54 |
1:00.39 |
-8.15 |
| 11 |
Paxton, Jack E (14)
|
AMBPR |
1:07.91 |
1:06.19 |
-1.72 |
| 12 |
Sundaresh Babu, Riswanth Har (14)
|
AMBPR |
1:24.28 |
1:21.07 |
-3.21 |
|
Nadimpalli, Roshith S (14)
|
AMPEAQ |
1:24.97 |
NS |
- |