| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Baker, Alanna C (7)
|
AMMLAC |
48.27 |
46.84 |
-1.43 |
| 2 |
Opitz, Lillian K (7)
|
AMPEAQ |
47.67 |
48.50 |
0.83 |
| 3 |
Schomaker, Charlotte J (7)
|
AMNHA |
51.27 |
50.36 |
-0.91 |
| 4 |
Lawrence, Rory C (7)
|
AMNHA |
53.54 |
52.05 |
-1.49 |
| 5 |
Obsincs, Gabrielle A (7)
|
AMNHA |
54.95 |
53.32 |
-1.63 |
| 6 |
McGuire, Addison F (7)
|
AMPEAQ |
1:00.19 |
53.88 |
-6.31 |
| 7 |
Brunner, Tessa G (7)
|
AMPEAQ |
54.75 |
54.32 |
-0.43 |
| 8 |
Capp, Molly L (7)
|
AMMOON |
54.68 |
54.37 |
-0.31 |
| 9 |
McKinney, Bailey R (7)
|
AMRACE |
1:00.16 |
55.31 |
-4.85 |
| 10 |
Hollenbeck, Mackenzie E (7)
|
AMANSC |
1:01.69 |
56.29 |
-5.40 |
| 11 |
Cegan, Olivia R (7)
|
AMHD |
58.37 |
58.97 |
0.60 |
| 12 |
Oliver, Emma E (7)
|
AMKW |
1:00.02 |
1:02.38 |
2.36 |
| 13 |
Robinson, Gabrielle D (7)
|
AMSFAC |
1:15.28 |
1:07.31 |
-7.97 |
| 14 |
Page, Brynley J (7)
|
AMHD |
1:14.55 |
1:08.59 |
-5.96 |
| 15 |
Chandiran, Sai Anshika (7)
|
AMPEAQ |
1:08.58 |
1:09.39 |
0.81 |
| 16 |
Radomski, Annabelle M (7)
|
AMHD |
1:19.28 |
1:10.57 |
-8.71 |
| 17 |
Berke, Mikayla R (7)
|
AMHD |
1:36.36 |
1:15.36 |
-21.00 |
|
Rees, Leia K (7)
|
AMNHA |
51.63 |
DQ |
- |
|
Hartman, Caroline R (7)
|
AMPEAQ |
1:09.32 |
DQ |
- |
|
Ressler, Shyloh W (7)
|
AMPRA |
1:25.67 |
NS |
- |
|
Palkovitch, Scarlet M (7)
|
AMRACE |
1:20.00 |
NS |
- |
|
Ponder, Alacia M (7)
|
AMRACE |
1:06.00 |
NS |
- |