| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Bhatia, Isabel A (12)
|
NTATAC |
42.91 |
43.02 |
0.11 |
| 2 |
Graham, Riley G (12)
|
NTCOPS |
42.27 |
43.30 |
1.03 |
| 3 |
Botto, Caroline B (12)
|
NTATAC |
|
44.58 |
- |
| 4 |
Sureka, Neethi (11)
|
NTATAC |
45.64 |
45.47 |
-0.17 |
| 5 |
Stembridge, Emily D (11)
|
NTCOPS |
44.65 |
46.01 |
1.36 |
| 6 |
Wagema, Isis M (12)
|
NTATAC |
48.18 |
46.07 |
-2.11 |
| 7 |
Kohok, Aneesha (12)
|
NTCOPS |
44.41 |
47.03 |
2.62 |
| 8 |
Taylor, Morgan N (11)
|
NTCOPS |
|
47.13 |
- |
| 9 |
Torres Sanchez, Meghan L (12)
|
NTCOPS |
49.94 |
47.50 |
-2.44 |
| 10 |
Osman, Heba (11)
|
NTCOPS |
51.29 |
47.85 |
-3.44 |
| 11 |
Escutia Castaneda, Ariadna (11)
|
NTCOPS |
51.93 |
49.93 |
-2.00 |
| 12 |
Mandrell, Olivia J (11)
|
NTATAC |
49.24 |
49.94 |
0.70 |
| 13 |
Amberkar, Nidhi A (12)
|
NTCOPS |
49.45 |
50.81 |
1.36 |
| 14 |
Mentgen, Harper A (11)
|
NTATAC |
|
50.99 |
- |
| 15 |
Chavan, Aditi A (11)
|
NTCOPS |
53.26 |
52.38 |
-0.88 |
| 16 |
Chavan, Anvi A (11)
|
NTCOPS |
55.92 |
58.16 |
2.24 |
|
Morales, Naima M (12)
|
NTCOPS |
|
NS |
- |