| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Overfelt, William C (12)
|
FGTSA |
40.11 |
39.25 |
-0.86 |
| 2 |
Brunetti, Giuliano P (11)
|
FGPOMP |
40.57 |
40.01 |
-0.56 |
| 3 |
Wingold, Charles R (11)
|
FGHAFL |
43.31 |
40.14 |
-3.17 |
| 4 |
Martin, Richard J (12)
|
FGTSA |
42.02 |
40.46 |
-1.56 |
| 5 |
Santos, Jonas Y (11)
|
FGSFTL |
43.55 |
42.18 |
-1.37 |
| 6 |
Kibler, Ryan A (11)
|
FGPST |
42.75 |
42.65 |
-0.10 |
| 7 |
Diaz, Diego (12)
|
FGAZFL |
45.41 |
42.91 |
-2.50 |
| 8 |
Jordan, Cole a (11)
|
FGHAFL |
56.62 |
43.52 |
-13.10 |
| 9 |
Tan, Matthew W (11)
|
FGTSA |
44.80 |
44.33 |
-0.47 |
| 10 |
Mercado, Alec (12)
|
FGHAFL |
44.54 |
44.34 |
-0.20 |
| 11 |
Lamy, Shawn D (12)
|
FGSFTL |
53.00 |
44.57 |
-8.43 |
| 12 |
Cajuste, Joshua J (12)
|
FGTSA |
42.73 |
44.69 |
1.96 |
| 13 |
Famiano, Gianni M (11)
|
FGPOMP |
46.55 |
45.53 |
-1.02 |
| 14 |
Herrera, Alejandro (11)
|
FGYSF |
45.18 |
45.88 |
0.70 |
| 15 |
Baker, Kevin T (11)
|
FGYSF |
46.23 |
45.97 |
-0.26 |
| 16 |
McCrady, James H (12)
|
FGSFTL |
47.89 |
46.36 |
-1.53 |
| 17 |
Dawkins, Mekhi B (11)
|
FGTSA |
47.93 |
46.50 |
-1.43 |
| 18 |
Maftei, Tudor (11)
|
FGSF |
1:02.43 |
49.35 |
-13.08 |
| 19 |
Richards, Cale B (12)
|
FGPST |
58.01 |
49.36 |
-8.65 |
| 20 |
Doyle, Sean R (12)
|
FGSUN |
54.19 |
49.84 |
-4.35 |
| 21 |
Rodriguez-Mora, Noah (11)
|
FGSUN |
|
52.85 |
- |
| 22 |
Vardakis, Nikolas A (11)
|
FGSUN |
57.52 |
54.85 |
-2.67 |
| 23 |
Gill, Andres F (12)
|
FGHAFL |
|
1:05.63 |
- |
|
Arbelo, Tyler A (11)
|
FGSF |
50.00 |
DQ |
- |
|
Szabo, Marcus (11)
|
FGPOMP |
42.28 |
DQ |
- |
|
Scott, Liam M (11)
|
FGPOMP |
55.34 |
DQ |
- |
|
Hayman, Christopher J (12)
|
FGHAFL |
|
DQ |
- |
|
Podiapolski, Dennis (11)
|
FGCCPR |
1:00.64 |
DQ |
- |
|
Givskov, Daniel (11)
|
FGSFTL |
47.33 |
NS |
- |
|
Calero, Fernando J (11)
|
FGCSSC |
52.40 |
NS |
- |
|
Garcia, Antonio A (12)
|
FGPOMP |
|
NS |
- |
|
Ream, Matteo F (12)
|
FGAZFL |
48.30 |
NS |
- |
|
Gautier, Oliver r (12)
|
FGHAFL |
|
NS |
- |