| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Braun, Gretchen L (9)
|
ADNTS |
31.29 |
31.82 |
0.53 |
| 2 |
Barkley, Katherine E (10)
|
ADNTS |
|
36.40 |
- |
| 3 |
Rochefort, Sydney E (9)
|
ADNTS |
36.83 |
39.36 |
2.53 |
| 4 |
Sanderson, Skyler G (9)
|
ADNTS |
40.65 |
40.10 |
-0.55 |
| 5 |
Bergen, Isabella R (10)
|
ADTBS |
49.44 |
44.39 |
-5.05 |
| 6 |
Law, Brenna M (9)
|
ADTBS |
50.50 |
44.68 |
-5.82 |
| 7 |
Andreescu, Stephanie (10)
|
ADNTS |
|
45.07 |
- |
| 8 |
Parker, Gracen M (9)
|
ADNTS |
|
46.32 |
- |
| 9 |
Renwick, Paige G (9)
|
ADTBS |
|
47.92 |
- |
| 10 |
Melby, Anna L (9)
|
ADTBS |
1:01.13 |
50.22 |
-10.91 |
| 11 |
Worthley, Hannah M (9)
|
ADNTS |
|
53.60 |
- |
| 12 |
Thompson, Hailey M (10)
|
ADNTS |
|
54.20 |
- |
| 13 |
Finnegan, Kate A (9)
|
ADNTS |
|
1:00.99 |
- |