| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Braun, Julia L (13)
|
ADNTS |
26.76 |
26.95 |
0.19 |
| 2 |
McCarthy, Ella I (13)
|
ADNHA |
27.79 |
27.16 |
-0.63 |
| 3 |
Wultsch-Fuller, Sonja E (13)
|
ADNTS |
26.94 |
27.30 |
0.36 |
| 4 |
Cordick, Helena E (14)
|
ADWAVE |
29.21 |
29.42 |
0.21 |
| 5 |
White, Nicole E (14)
|
ADNTS |
29.26 |
29.64 |
0.38 |
| 6 |
Rio, Willa K (13)
|
ADNHA |
29.68 |
29.66 |
-0.02 |
| 7 |
Fernandez-Turo, Rocio A (13)
|
ADNHA |
30.68 |
30.24 |
-0.44 |
| 8 |
Camarda, Avery H (14)
|
ADBENN |
31.11 |
32.07 |
0.96 |
| 9 |
Cohen, Evyn B (14)
|
ADNHA |
|
32.82 |
- |
| 10 |
Lu, Ziya (14)
|
ADNTS |
|
32.89 |
- |
| 11 |
Melnikova, Alexandra (13)
|
ADNTS |
37.01 |
33.00 |
-4.01 |
| 12 |
Budhu, Sonia M (13)
|
ADNHA |
37.72 |
35.48 |
-2.24 |
| 13 |
Grant, Adeline K (13)
|
ADNHA |
|
35.60 |
- |
| 14 |
Gioppo, Abigail M (13)
|
ADNHA |
40.92 |
39.05 |
-1.87 |