| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Braun, Gretchen L (9)
|
ADNTS |
38.26 |
37.69 |
-0.57 |
| 2 |
McIntosh, Saige E (9)
|
ADTBS |
43.02 |
40.32 |
-2.70 |
| 3 |
Barkley, Katherine E (10)
|
ADNTS |
|
45.23 |
- |
| 4 |
Rochefort, Sydney E (9)
|
ADNTS |
43.68 |
45.69 |
2.01 |
| 5 |
Sanderson, Skyler G (9)
|
ADNTS |
49.22 |
48.03 |
-1.19 |
| 6 |
Bergen, Isabella R (10)
|
ADTBS |
52.25 |
48.11 |
-4.14 |
| 7 |
Renwick, Paige G (9)
|
ADTBS |
|
56.25 |
- |
| 8 |
Andreescu, Stephanie (10)
|
ADNTS |
|
1:00.16 |
- |
| 9 |
Worthley, Hannah M (9)
|
ADNTS |
|
1:00.44 |
- |
| 10 |
Finnegan, Kate A (9)
|
ADNTS |
|
1:03.53 |
- |
|
Parker, Gracen M (9)
|
ADNTS |
|
DQ |
- |
|
Thompson, Hailey M (10)
|
ADNTS |
1:05.56 |
DQ |
- |