| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Clark, Campbell U (12)
|
MRHVD |
26.72 |
28.04 |
1.32 |
| 2 |
Sarpong, Afia (11)
|
MRHVD |
29.19 |
29.65 |
0.46 |
| 3 |
Pece, Delaney R (11)
|
ADMDST |
30.24 |
29.89 |
-0.35 |
| 4 |
Vos, Brooke C (11)
|
ADMDST |
31.60 |
30.08 |
-1.52 |
| 5 |
Hannon, Morgan A (11)
|
MRHVD |
30.51 |
30.89 |
0.38 |
| 6 |
Dabo, Mariama F (11)
|
MRHVD |
28.43 |
31.11 |
2.68 |
| 7 |
Horgan, Rosemary E (11)
|
MRHVD |
30.09 |
31.73 |
1.64 |
| 8 |
Nemeth, Ava E (11)
|
MRHVD |
34.23 |
33.02 |
-1.21 |
| 9 |
Jarvis, Cassandra D (12)
|
MRHVD |
32.53 |
34.49 |
1.96 |
| 10 |
Bautista, Jasmine (12)
|
MRUN |
38.27 |
34.97 |
-3.30 |
| 11 |
Jarvis, Emma M (12)
|
MRHVD |
37.09 |
38.08 |
0.99 |
| 12 |
Perez Quiroz, Brianna Y (12)
|
MRUN |
40.42 |
38.42 |
-2.00 |
| 13 |
Cabrera Zavala, Alexa M (11)
|
MRUN |
42.72 |
40.83 |
-1.89 |
| 14 |
Harrell, Aya (11)
|
MRUN |
48.97 |
44.74 |
-4.23 |
| 15 |
Zeman, Sophia E (11)
|
MRHVD |
43.57 |
46.57 |
3.00 |
|
Huang, Alyssa (11)
|
MRCFJV |
32.51 |
NS |
- |