| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Kelly, Hannah E (10)
|
OHLTPY |
39.49 |
39.45 |
-0.04 |
| 2 |
Newcomb, Cassidy N (10)
|
OHLTPY |
39.93 |
39.58 |
-0.35 |
| 3 |
Scott, Caroline G (10)
|
OHLTPY |
41.03 |
42.06 |
1.03 |
| 4 |
McDonald, Lily P (10)
|
OHLTPY |
42.93 |
43.38 |
0.45 |
| 5 |
Harrison-Mills, Auden M (10)
|
OHLTPY |
44.08 |
43.99 |
-0.09 |
| 6 |
Wolfe, Emily E (10)
|
OHLTPY |
|
48.31 |
- |
| 7 |
McDonald, Holly M (9)
|
OHLTPY |
49.44 |
50.58 |
1.14 |
| 8 |
Wilkerson, Oliviah J (10)
|
OHLTPY |
58.33 |
55.05 |
-3.28 |
| 9 |
Bernard, Emily O (10)
|
OHLTPY |
58.69 |
55.33 |
-3.36 |
| 10 |
Amadu, Amani (9)
|
OHCNYW |
|
1:01.96 |
- |
|
Williams, Jaliyah (9)
|
OHCNYW |
|
NS |
- |